The Neuroimmunology team is seeking a motivated and technically skilled scientist with strong CNS cell biology expertise to join our Cambridge-based research group. This role will focus on culturing stable cell lines, primary and iPS-derived microglia, and other CNS models to investigate disease-associated functions and phenotypes that support the identification and validation of novel therapeutic targets for neurodegenerative diseases.
The ideal candidate will have a strong foundation in cell culture, microscopy, and functional assays, along with the ability to analyze and interpret data independently. This position offers the opportunity to collaborate with experts across genetics, chemical biology, immunology, and pharmacology in a dynamic and multidisciplinary research environment.
Key Responsibilities
- Culture stable cell lines, primary rodent neurons and glia (e.g., microglia), and human iPS-derived CNS cell models including microglia and neurons
- Apply standard sterile techniques and perform cell transfections using lipid-based and electroporation methods
- Perform cell staining (immunofluorescence, cell masks, and structural stains)
- Acquire high-quality images and maintain strong microscopy techniques
- Utilize knowledge of mouse neuroanatomy for experimental design and interpretation
- Assist in the development and execution of disease-relevant cell-based functional assays
- Perform high content imaging, biochemical, and molecular profiling techniques (e.g., protein/RNA extraction, Western blot, ELISA, qPCR)
- Analyze data independently, including statistical evaluation of results
- Participate in method optimization and troubleshooting activities
- Communicate results clearly in lab meetings and small team settings
Skills & Qualifications
Required
- BS/BA in Biology, Neuroscience, Molecular Biology, Bioengineering, Biotechnology, or related discipline
- At least 2+ years of relevant industry or academic laboratory experience
- Strong knowledge of cell culture methods and sterile technique
- Experience with immortalized cell lines, primary rodent CNS cells, and human iPS-derived CNS cell models
- Proficiency in transfection techniques (lipid-based and electroporation)
- Proven microscopy skills and experience in image acquisition
- Understanding of cell staining methods and structural imaging
- Ability to perform independent data analysis with practical statistical knowledge
- Strong critical thinking, problem-solving, and troubleshooting skills
Preferred
- Experience with plate-based high content imaging microscopy and live cell imaging
- Proficiency in quantitative image analysis
- Familiarity with cellular signaling and functional studies (e.g., phagocytosis, cell motility)
- Working knowledge of biochemical and molecular biology techniques (protein/RNA extraction, quantitation, detection)
- Strong oral, written, and presentation skills
Education
- BS/BA in relevant scientific discipline required (advanced degree preferred but not mandatory)
