The Merck Functional Genomics team, within the Department of Data, AI, and Genome Science (DAGS), is seeking a passionate Cell Biologist for a CRISPR Screening Contractor position in Cambridge, MA. This role is at the forefront of drug discovery, utilizing high-throughput assays and innovative screening methods—including CRISPR, Perturb-seq, and Spatial Transcriptomics—to identify and validate new therapeutic targets. You will collaborate closely with specialists in cardiometabolic disease, immunology, and neuroscience to impact all stages of the drug development pipeline, from pre-clinical discovery through clinical development.
Responsibilities
- Develop, execute, and troubleshoot new cell-based assays and analysis methods to validate drug targets across multiple therapeutic areas.
- Execute large-scale CRISPR screens in both pooled and arrayed formats to drive genomic discovery.
- Work with complex cellular models, including primary cells, iPSCs, and three-dimensional models, in addition to immortalized cell lines.
- Apply advanced techniques such as lentiviral transduction, RNP nucleofection for gene editing, and high-content imaging.
- Utilize lab automation, liquid handling technologies, and flow cytometry to streamline high-throughput screening workflows.
- Perform Next-Generation Sequencing (NGS) and single-cell sequencing to analyze experimental outcomes.
- Design and execute experiments, interpret complex data sets, and communicate findings clearly to teams and stakeholders.
- Maintain meticulous lab notebooks and detailed records of all experimental work.
- Participate actively in the scientific dialogue of the group, contributing to target ID and validation strategies.
- Manage time effectively to maintain a high level of productivity in a fast-paced, 90% lab-based environment.
Education
- PhD in molecular and cell biology or related disciplines; OR
- BS/MS degree with 5+ years of post-graduate wet lab research experience.
Experience
- Demonstrated expertise with mammalian cell culture using sterile techniques and cell-based assay development.
- Hands-on experience with CRISPR-screening is required.
- Experience or a strong motivation to learn RNP nucleofection, high-content imaging, and flow cytometry.
- Familiarity with liquid handling automation and NGS/Single Cell Sequencing workflows.
Additional Information
- Location: 100% on-site at the Research Lab in Cambridge, MA
- Work Commitment: This position requires approximately 90% of the time to be spent physically in the lab.
- Environment: Ideal for a proactive collaborator who can work independently while contributing to a multi-disciplinary scientific team.
